JMIRx Bio

The prediction of tertiary RNA structures is significant to the field of medicine (eg, messenger RNA [mRNA] vaccines, genome editing) and the exploration of viral transcripts. Though many RNA folding software programs exist, few studies have condensed their locus of attention solely to viral pseudoknotted RNA. These regulatory pseudoknots play a role in genome replication, gene expression, and protein synthesis.

People with type 1 diabetes (T1D) depend on external insulin to regulate their blood glucose level (BGL) within the normoglycemic range between 4.0 to 7.0 mmol/L. Patients with T1D routinely conduct self-monitoring of blood glucose through finger pricks before insulin injections. An artificial pancreas is an innovative device that mimics the function of a healthy pancreas. Despite its recent advancement, the control algorithms used in an artificial pancreas are still lagging in delivering the proper insulin dosage to patients with T1D. Previous researchers attempted to improve the interrelation between parameters and variables in the original Hovorka equations model, later known as the improved Hovorka equations model; however, the improved equations model has not been tested in terms of its usability to regulate and control the BGL in a safe range for 2 or more people with T1D.

The culture of immortal cell lines has become an indispensable tool in the field of modern biotechnology and has been used in the production of human and viral veterinary vaccines, therapeutic recombinant proteins, interferons, and monoclonal antibodies. Several approaches are used to immortalize cells in culture, such as transduction of cells with viral oncogenes, induced expression of telomerase reverse transcriptase, and spontaneous immortalization by serial passage of primary cell lines.