The manuscript [1] presents a well-structured and novel study exploring the correlation between seed coat color and the optimal concentration of 6-benzylaminopurine (BAP) for shoot regeneration in leaf lettuce cultivars. The research is timely and addresses a significant challenge in plant tissue culture—genotypic variability in regeneration efficiency.

The experimental design is solid, involving 6 cultivars with distinct seed coat colors.

The use of the CIELAB color scale adds objectivity to phenotypic assessments.

The identification of seed coat color as a potential morphological marker for shoot regeneration efficiency is innovative and potentially valuable for breeding and transformation programs.

Language and clarity: While the scientific content is strong, the manuscript would benefit from careful language editing for grammar and fluency.

Statistical reporting: The statistical significance (eg, P values) is noted, but a more detailed description of the statistical models and effect sizes would enhance reproducibility.

Figures and tables: Ensure that all figures and tables referenced (eg, Figure 1, Table S1) are clearly labeled and formatted for clarity. Including a visual summary (graphical abstract) could further enhance impact.

Discussion depth: The discussion of mechanisms linking seed coat pigmentation to shoot regeneration could be expanded, possibly integrating flavonoid biosynthesis and tissue culture responsiveness more.

Conclusion: Consider sharpening the Conclusion to emphasize the practical applications of the findings, especially in the context of lettuce transformation systems.

Overall, this is a meaningful contribution to plant biotechnology literature and warrants publication after minor revisions.