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1. “Material and methods - Sampling”: For the tick sampling in this manuscript, what method did you used in this study (ie, how did you decide which tick samples to include and which samples to exclude)? How did you ensure that the sample is representative of the true distribution of ticks in the study area? The distribution and frequency estimates from this sample might not be extended to the whole tick population if the sampling is biased.
2. “Material and methods - Sampling”: Could the author provide the rationale or justification of the choice of “
3. “Discussion”: I really like the analysis of seasonal trend presented in
1. Line 41: Spell out “$.”
2. Line 44: Remove “(4)”—duplicated reference number.
3. Line 51: Remove “(9)”—duplicated reference number.
4. Line 85, “valid diagnostic keys”: Could the author be more specific about the “diagnostic key” being used? Adding a sentence to briefly describe the key would be great.
5. Lines 147-151, “two professional stereo microscopes...in the entomological research”: This part should belong to Methods section.
Thank you, author, for addressing all my comments and making all necessary changes to the manuscript. I do not have any more comments.
None declared.