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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">JMIRxBio</journal-id>
      <journal-id journal-id-type="nlm-ta">JMIRx Bio</journal-id>
      <journal-title>JMIRx Bio</journal-title>
      <issn pub-type="epub">2819-2044</issn>
      <publisher>
        <publisher-name>JMIR Publications</publisher-name>
        <publisher-loc>Toronto, Canada</publisher-loc>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">v3i1e71626</article-id>
      <article-id pub-id-type="pmid"/>
      <article-id pub-id-type="doi">10.2196/71626</article-id>
      <article-categories>
        <subj-group subj-group-type="heading">
          <subject>Peer-Review Report</subject>
        </subj-group>
        <subj-group subj-group-type="article-type">
          <subject>Peer-Review Report</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Peer Review of “Effects of Ventral Pallidum–Nucleus Accumbens Shell Neural Pathway Modulation on Sucrose Consumption and Motivation in Female Rats: Chemogenetic Manipulation Study”</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="editor">
          <name>
            <surname>Singh</surname>
            <given-names>Om</given-names>
          </name>
        </contrib>
      </contrib-group>
      <contrib-group>
        <contrib id="contrib1" contrib-type="author" corresp="no">
          <name name-style="western">
            <surname>Wirtshafter</surname>
            <given-names>David</given-names>
          </name>
          <degrees>BA, MA, PhD</degrees>
          <xref rid="aff1" ref-type="aff">1</xref>
        </contrib>
      </contrib-group>
      <aff id="aff1">
        <label>1</label>
        <institution>The University of Illinois at Chicago</institution>
        <addr-line>Chicago, IL</addr-line>
        <country>United States</country>
      </aff>
      <pub-date pub-type="collection">
        <year>2025</year>
      </pub-date>
      <pub-date pub-type="epub">
        <day>8</day>
        <month>3</month>
        <year>2025</year>
      </pub-date>
      <volume>3</volume>
      <elocation-id>e71626</elocation-id>
      <history>
        <date date-type="received">
          <day>22</day>
          <month>1</month>
          <year>2025</year>
        </date>
        <date date-type="accepted">
          <day>22</day>
          <month>1</month>
          <year>2025</year>
        </date>
      </history>
      <copyright-statement>©David Wirtshafter. Originally published in JMIRx Bio (https://bio.jmirx.org), 08.03.2025.</copyright-statement>
      <copyright-year>2025</copyright-year>
      <license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/">
        <p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIRx Bio, is properly cited. The complete bibliographic information, a link to the original publication on https://bio.jmirx.org/, as well as this copyright and license information must be included.</p>
      </license>
      <self-uri xlink:href="https://bio.jmirx.org/2025/1/e71626" xlink:type="simple"/>
      <self-uri xlink:href="https://bio.jmirx.org/2025/1/e71627" xlink:type="simple"/>
      <related-article related-article-type="companion" id="preprint" ext-link-type="doi" xlink:href="10.1101/2024.11.05.622115" vol="1" page="622115" xlink:title="Preprint (bioRxiv):" xlink:type="simple">https://www.biorxiv.org/content/10.1101/2024.11.05.622115v1</related-article>
      <related-article related-article-type="companion" id="v3i1e71629" ext-link-type="doi" xlink:href="10.2196/71629" vol="3" page="e71629" xlink:title="Authors' Response to Peer-Review Reports:" xlink:type="simple">https://bio.jmirx.org/2025/1/e71629/</related-article>
      <related-article related-article-type="companion" id="v3i1e68519" ext-link-type="doi" xlink:href="10.2196/68519" vol="3" page="e68519" xlink:title="Published Article:" xlink:type="simple">https://bio.jmirx.org/1/e68519/</related-article>
      <kwd-group>
        <kwd>ventral pallidum</kwd>
        <kwd>nucleus accumbens shell</kwd>
        <kwd>chemogenetics</kwd>
        <kwd>sucrose</kwd>
        <kwd>feeding behavior</kwd>
        <kwd>food motivation</kwd>
        <kwd>palatable food</kwd>
        <kwd>DREADD</kwd>
        <kwd>designer receptors exclusively activated by designer drugs</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <disp-quote>
      <p>This is a peer-review report submitted for the paper “Effects of Ventral Pallidum–Nucleus Accumbens Shell Neural Pathway Modulation on Sucrose Consumption and Motivation in Female Rats: Chemogenetic Manipulation Study.”</p>
    </disp-quote>
    <sec>
      <title>Round 1 Review</title>
      <sec>
        <title>General Comments</title>
        <p>In this paper [<xref ref-type="bibr" rid="ref1">1</xref>], the authors present an interesting and well-written paper dealing with the effects of stimulation and inhibition of projections from the ventral pallidum to the nucleus accumbens shell on feeding and food reinforced behaviors. The methods used are cutting edge, and my comments and suggestions are relatively minor.</p>
      </sec>
      <sec>
        <title>Specific Comments</title>
        <sec>
          <title>Minor Comments</title>
          <p>1. In the third paragraph of the Introduction, the sentence beginning with “Parallelly” is very awkward; I am sure there is a way to word this that does not use “parallelly.” Also, the previous sentence could be made clearer as to whether effects on sucrose consumption are found just in female rats.</p>
          <p>2. The number of subjects should be listed in the Methods.</p>
          <p>3. In the last paragraph of the body of the manuscript, the sentence beginning with “The discrepancies observed across studies of this pathway...” is unfinished, and I am uncertain what the authors intended to say.</p>
          <p>4. In discussing the differences between the results observed here and those reported by Vanchez et al [<xref ref-type="bibr" rid="ref2">2</xref>], is it possible that these may reflect the use of “closed-loop” manipulations linked to the occurrence of licking in the Vanchez et al [<xref ref-type="bibr" rid="ref2">2</xref>] paper, in contrast to the continuous modulation produced here by the use of the DREADD (designer receptors exclusively activated by designer drugs) technique? Also, in this section, the authors could be a bit clearer as to why the techniques used by Vanchez et al [<xref ref-type="bibr" rid="ref2">2</xref>] would be expected to label a different subpopulation of cells than was the case in this study.</p>
        </sec>
      </sec>
    </sec>
  </body>
  <back>
    <app-group/>
    <glossary>
      <title>Abbreviations</title>
      <def-list>
        <def-item>
          <term id="abb1">DREADD</term>
          <def>
            <p>designer receptors exclusively activated by designer drugs</p>
          </def>
        </def-item>
      </def-list>
    </glossary>
    <fn-group>
      <fn fn-type="conflict">
        <p>None declared.</p>
      </fn>
    </fn-group>
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</article>
